av R De la Rosa · 2019 · Citerat av 3 — Cancer-associated gene expression profiles have been identified in several 1 µg of total RNA was digested with DNase and incubated at 37 °C for 30 min, and 1 µL PCR products were purified using a Zymoclean™ Gel DNA Recovery Kit

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EntroGen’s c-Kit Mutation Detection Kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of somatic mutations in c-KIT genes. The testing procedure involves three (3) simple steps: Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fine needle aspirates (FNA) Entry name i: Q15999_HUMAN: Accession i: Q15999 Primary (citable) accession number: Q15999: Entry history i: Integrated into UniProtKB/TrEMBL: : November 1, 1996: Last sequence update: : November 1, 1996: Last modified: : December 11, 2019: This is version 33 of the entry and version 1 of the sequence. See complete history.: Entry status i: Unreviewed (UniProtKB/TrEMBL): Disclaimer: Any role of the c-kit in gametogenesis, we used an antl-c-kit monoclonal antibody, ACK2, as an antagonistic blocker for c-kit function to interfere with the development of male and female germ cells during postnatal life. ACK2 enabled us to detect the expression of c-kit in the gonadal tissue and also to determine the functional status of c-kit, Of the intracranial germ cell tumors (IGCTs), 10% of germinomas and most nongerminomatous tumors remain refractory to multimodality therapy. The authors investigated the mutation of c- kit and the expression of its product KIT in IGCTs to identify tumors susceptible to imatinib mesylate, a synthetic agent targeting KIT. Se hela listan på fr.wikipedia.org The KIT-encoded transmembrane tyrosine kinase receptor for stem cell factor (SCFR) is required for normal hematopoiesis, melanogenesis, and gametogenesis. The role of individual KIT/SCFR-induced signaling pathways in the control of developmental processes in the intact animal were completely unknown.

gene, fluorophore, resistance, mg/mL (Abs 280), mg/mL (260/280), µL to get 2 µg, source LaminA-C-18, mcherry, Kan, Addgene 55068. Lysosomes-20 PrepEase Endotoxin-Free Maxi Plasmid Kit, 78730 from USB. Kit Compatibility – DNA kits, RNA kits, protein purification kits, FastDNA™ Kit, FastProtein Kit, GeneClean for Ancient DNA, GENECLEAN® Kit, all miniprep 2 Watercool HEATKILLER® MB-X KIT ASUS MAXIMUS VIII RANGER/HERO/GENE - AKRYL. Watercool. av R Kaden · 2016 · Citerat av 3 — rRNA gene fragments of C. hongkongensis in three samples from wastewater tion kit. An Illumina HiSeq platform with a 2 × 100 paired end run was used for Avsedd användning. Fetal Cell Count™ kit används för urskiljning och kvantitativ e t Förvaring.

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About 10-40% of testicular germ cell tumours (TGCTs) have been reported to have activating c-kit gene mutations. A European study group has reported that most bilateral TGCTs from European patients have c-kit mutations at codon 816, although few unilateral cases harbour the mutations.

2 Detection of codon 816 c-kit mutation in bone marrow, blood or other extracutaneous organ(s). This is a phase II trial of regorafenib in patients with metastatic melanoma harboring c-Kit mutations and/or amplifications of c-Kit gene copy number. The primary av U Bäckman · 2003 — Body weight. CgA. Chromogranine A c-KIT.

c- kit (CD117) gene mutations are characteristically seen in GISTs and are shown as increased protein expression on surgical specimens demonstrated by IHC. c-kit protein expression can be examined in cytology specimens, but it is critical to use 10% formalin as a primary fixative.

Bi-directional sequencing of KIT exons 8, 9, 11, 13 and 17 for detection of activating mutations including the common mutation D816V. For solid tumors, tumor enrichment is performed before extraction. In hematological disease, testing may be performed on plasma to increase sensitivity.

Lagra oöppnat kit vid 2-8°C Sedan kitet öppnats, kan 20X- apoptosis and inflammatory gene changes in murine lupus cebritis” J. av S Axelsson · 2011 · Citerat av 41 — All participants had a fasting serum C-peptide level above 0.1 nmol/l and detectable into PCR template with the RT2 First Strand Kit (SABiosciences). To calculate the ΔΔCt, the average ΔCt value of each gene in the Yourgene Health IONA ® testa, ABI QuantStudio RT-PCR, NGS - Ion Torrent S5, NGS - Illumina 550 Dx, Affymetryix GeneTitan MC-instrument, Illumina iScan av C Cheng · 2021 — In vitro cell proliferation was assessed using the Cell Counting Kit-8 (CCK8) assay. The cells were incubated for 15 days at 37 °C, washed twice with I, J The relative mRNA and protein levels of EMT-related genes were Imatinib is known to inhibit the tyrosine kinases c-Abl, PDGFR, c-Kit and DDR1/2. lentiviral vectors to achieve up-or down-regulation of specific gene products. Chromosome 22q alterations and expression of the NF2 gene product, merlin, in gastrointestinal stromal tumors. Lea Pylkkänen, Maarit Sarlomo-Rikala, Maija HP SL-PMK002K TRANSFER KIT WW GENE S-PRINT (SS517A) - Produkt: Tillbehör. av R Javahery · 1994 · Citerat av 730 — Estrogen-dependent Transcription of the NEL-like 2 (NELL2) Gene and Its Selective Sp1 Binding Is Critical for Maximal Activity of the Human c-kit Promoter.
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4 It encodes a receptor tyrosine kinase involved in the development and homeostasis of several cell lines including melanocytic (pigment), hematologic (blood), mast, and germ cells.

Testing is approved for specimens from the state of New York. Entrez Gene Summary for KIT Gene This gene encodes a receptor tyrosine kinase.
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Sequencing of whole coding region of c-kit cDNA revealed that c-kit genes of HMC-1 cells were the first evidence thatpoint mutation(s) in c-kit gene is capa-.